Journal: International Journal of Molecular Sciences
Article Title: Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Enhance Chondrocyte Function by Reducing Oxidative Stress in Chondrocytes
doi: 10.3390/ijms26167683
Figure Lengend Snippet: Effect of UCMSC-EVs on oxidative stress of chondrocytes. Chondrocytes were treated with UCMSC-EVs at concentrations of 0 (Control group) or 10 8 –10 10 particles/mL (UCMSC-EVs group) for 7 days to assess oxidative stress. ( A ) Mitochondrial superoxide levels were evaluated by quantifying MitoSOX Red fluorescence intensity ( n = 6). ( B , C ) Protein expression levels of SOD-2 and Sirt-3 were analyzed by Western blotting following treatment with 10 10 particles/mL UCMSC-EVs. β-actin was used as the internal control. Protein expression data are normalized to the Control group (defined as 1) and presented as mean ± standard error of the mean (SEM; n = 3–5). * and ** indicate p < 0.05 and p < 0.01, respectively, compared to the chondrocytes in the Control group.
Article Snippet: The antibodies used in this study are as follows: Superoxide Dismutase 2 (SOD-2) (cat. no. 24127-1-AP, proteintech; 1:5000), Sirt-3 (cat. no. 12186-1-AP, proteintech; 1:1000), and β-actin (cat. no. A5441, Sigma).
Techniques: Control, Fluorescence, Expressing, Western Blot